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Cytotoxicity & LD 50


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5 replies to this topic

#1 Jen55❤

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Posted 06 February 2013 - 09:19 AM

Hi folks. I am doing a cytotoxicity assay on human lymphocytes. I am using two different test substances (of my choosing) and I am going to run my cells in a 96 well plate through a plate reader. This program will give me absorbacne values that i am supposed to put into excel and generate a graph. How do I get a lethal dose from there? Any help would be appreciated. Also, am I plotting absorbacne with time or some other parameter/variable?

Edited by Jen55❤, 06 February 2013 - 03:44 PM.


#2 bob1

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Posted 06 February 2013 - 11:28 AM

OK - if you think about this a little bit, you will see the answer is pretty obvious.

To determine a lethal dose, what is it that has to change over your assay plate?

#3 bob1

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Posted 06 February 2013 - 04:33 PM

Yup, so it is the dose of drug that you have to change.

#4 Jen55❤

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Posted 07 February 2013 - 10:26 AM

Yesterday was a long day of brain malfunction. Yes, thank you for the answer...drug concentration.

#5 Jen55❤

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Posted 07 February 2013 - 10:31 AM

So my class doesn't have a multi well pipettes. Is there any advice/techniques to plate without causing contamination/pipettes errors?

#6 bob1

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Posted 07 February 2013 - 11:19 AM

Change tips between each well and look to check that you are sucking up (and dispensing) appropriate amounts of the things you are adding. You can also try reverse pipetting.




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