5 replies to this topic

[Jen55❤]

Hi folks. I am doing a cytotoxicity assay on human lymphocytes. I am using two different test substances (of my choosing) and I am going to run my cells in a 96 well plate through a plate reader. This program will give me absorbacne values that i am supposed to put into excel and generate a graph. How do I get a lethal dose from there? Any help would be appreciated. Also, am I plotting absorbacne with time or some other parameter/variable?

Edited by Jen55❤, 06 February 2013 - 03:44 PM.

[bob1]

OK - if you think about this a little bit, you will see the answer is pretty obvious.

To determine a lethal dose, what is it that has to change over your assay plate?

[bob1]

Yup, so it is the dose of drug that you have to change.

[Jen55❤]

Yesterday was a long day of brain malfunction. Yes, thank you for the answer...drug concentration.

[Jen55❤]

So my class doesn't have a multi well pipettes. Is there any advice/techniques to plate without causing contamination/pipettes errors?

[bob1]

Change tips between each well and look to check that you are sucking up (and dispensing) appropriate amounts of the things you are adding.  You can also try reverse pipetting.