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determining the amount of lyticase to use to break open conidia from tissue swab

Conidia cell lysis lyticase or zymolase

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#1 MyluWarrior

MyluWarrior

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Posted 06 February 2013 - 06:14 AM

I am new here, so I hope I posted this in the right place. I am developing a protocol to extract DNA for positive identification of fungal conidia from tissue swab samples. I want to use lyticase or zymolase. My question is how do I determine the concentration of lyticase or zymolase to use? I am planning on pressing the swabs against the sides of an ep tube with maybe 750 to 1000 ul of the enzyme in solution then incubating for a while. I don't expect there to be a ton of conidia on the swabs; we just need to determine the presence of the fungi using PCR.

Thanks,
Jim




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