Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

determining the amount of lyticase to use to break open conidia from tissue swab

Conidia cell lysis lyticase or zymolase

  • Please log in to reply
No replies to this topic

#1 MyluWarrior



  • Members
  • Pip
  • 1 posts

Posted 06 February 2013 - 06:14 AM

I am new here, so I hope I posted this in the right place. I am developing a protocol to extract DNA for positive identification of fungal conidia from tissue swab samples. I want to use lyticase or zymolase. My question is how do I determine the concentration of lyticase or zymolase to use? I am planning on pressing the swabs against the sides of an ep tube with maybe 750 to 1000 ul of the enzyme in solution then incubating for a while. I don't expect there to be a ton of conidia on the swabs; we just need to determine the presence of the fungi using PCR.


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.