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Continuous growth of E. coli on AMP LB plate

e. coli lawn amp

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#1 tihong10

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Posted 05 February 2013 - 09:55 AM

Hi my LB AMP plates were streaked with transformed E.coli and colonies don't grow. Rather this creamy continuous film like lawn grows. I wonder if this is transformed E. coli or not...Posted Image

#2 Salabasama

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Posted 05 February 2013 - 11:56 AM

It depends. If you streaked for isolation with a wire and ended up with a lawn, that's certainly mysterious. If you spread an aliquot of liquid with cells in it, then the lawn makes more sense. If that is the case, I suggest diluting your liquid sample with a bit of growth medium. Maybe try a few plates, at 1/10, 1/100, ect. Those would probably give distinct colonies.

#3 tihong10

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Posted 25 February 2013 - 06:43 PM

Yes, my plates were spreads of liquid aliquots...I will try your dilution suggestion...Thank you!





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