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Plasmid compatibly groups

plasmid pBBR pBR322 pMB1

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5 replies to this topic

#1 microfir

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Posted 05 February 2013 - 05:29 AM

Hi all,

I need to use 2 plasmid with different ori's ,pMB1 and pBBR1 based plasmids.


Are they compatible?


Couldn't find it over the web.


Thanks



#2 bob1

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Posted 05 February 2013 - 12:42 PM

Compatible for what?

#3 microfir

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Posted 05 February 2013 - 10:16 PM

With each other



#4 bob1

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Posted 06 February 2013 - 12:38 AM

for cloning? expression? growth?...

#5 microfir

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Posted 06 February 2013 - 01:03 AM

I would like to use pMB1 based plasmid for protein expression under arabinose inducible promoter . And pBBR1 plasmid that carries lacZ under control of a specific promoter that should to be activated by the inducible protein. Can both of these plasmids reside in E. coli at the same time ?


Thanks

#6 bob1

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Posted 06 February 2013 - 11:39 AM

Well, it seems that pMB is designed for eukaryotic expression, but it will surely replicate in bacteria without problems, I don't know about the expression from it in bacteria. pBBR seems like a normal sort of vector, so I don't see any problem there.

I think it will work, but your best bet is to try it and see.





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