Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Cryopreservation of MSC at lower cell density

MSC Cryopreservation

  • Please log in to reply
2 replies to this topic

#1 ChrisHarris



  • Active Members
  • Pip
  • 10 posts

Posted 04 February 2013 - 04:23 AM

Most publications suggest freezing mesenchymal stem cells at about 10^6 cells/ml of cryopreservative solution.

If I have very low cell numbers, my inclination is to store at below 10^6 cells/ml to ensure I'm not working with tiny volumes.

I have done a quick literature search & can't see anything - does anyone have a link to anything published about freezing at lower cell density?

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,546 posts

Posted 04 February 2013 - 11:48 AM

I don't work with stem cells, so this should be read with caution - many cells lines will survive well if frozen at lower densities, however, it does pay, when reviving the cells, to take this lower density into account and adjust the area onto which they are plated. For example, seed into a t-25 instead of a t-75.

#3 Tabaluga


    Making glass out of shards

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 434 posts

Posted 04 February 2013 - 12:03 PM

I don't work with MSCs either, but with a different kind of stem cells. I'd say it should be fine to freeze at lower density and I fully agree with bob that when you thaw your cells, you should plate them in a small flask first. Also take care to keep the time for thawing as short as possible and if they don't really seem to grow well in the first few days, just leaving them alone in the incubator for a while (lower frequency of medium exchange etc.) might be helpful.

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.