How long can transformation reactions be stored at 4 degrees? I'd like to transform NovaBlue competent cells (recover in 850uL SOC for 1 hour with shaking), and store them over the weekend to be plated Sunday evening. Any special steps I should take? Any recommendations? I was wondering if I could simply store the cell suspension/ liquid culture at 4 degrees or should I pellet the bacteria and store at -20? Should I just suck it up and go in Sunday night?
Thanks for any and all input!
Submit your paper to J Biol Methods today!
Storing NovaBlue Transformation Reactions in SOC
Started by Ihla, Feb 01 2013 08:10 AM
Transformation
1 reply to this topic
#2
bob1
-
- Global Moderators
-
- 4,893 posts
Thelymitra pulchella
292
Excellent
Excellent
Posted 01 February 2013 - 02:55 PM
-20 will kill the bugs (freeze/thaw) unless you make a glycerol stock, in which case -80 is better. You can store them for a couple of days at 4 deg C, but the number of bacteria that survive drops off quickly. It is always much much much better to do the plating immediately after the recovery.
Also tagged with one or more of these keywords: Transformation
 |
Protocols and Techniques Forums →
Molecular Cloning →
pcDNA 3.1 Directional TOPO clone issuesStarted by Jeremy55, 01 Dec 2013  ligation, transformation
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Problems with ligation and transformationStarted by Ater, 31 Oct 2013 ligation, Transformation
|
|
|
||
Protocols and Techniques Forums →
Molecular Biology →
ligation transformationStarted by student47, 11 Oct 2013 ligation, transformation
|
|
|
||
Protocols and Techniques Forums →
Molecular Biology →
Bacterial transformation problemsStarted by HRL10, 11 Oct 2013 Bacteria, transformation, E.coli and 2 more...
|
|
|
||
Protocols and Techniques Forums →
General Lab Techniques →
I dissolved the DNA in 1XTAE instead of TE buffer, will it affect ligation?Started by babethepig, 03 Oct 2013 DNA, ligation, transformation and 2 more...
|
|
|
