How long can transformation reactions be stored at 4 degrees? I'd like to transform NovaBlue competent cells (recover in 850uL SOC for 1 hour with shaking), and store them over the weekend to be plated Sunday evening. Any special steps I should take? Any recommendations? I was wondering if I could simply store the cell suspension/ liquid culture at 4 degrees or should I pellet the bacteria and store at -20? Should I just suck it up and go in Sunday night?
Thanks for any and all input!
0
Storing NovaBlue Transformation Reactions in SOC
Started by Ihla, Feb 01 2013 08:10 AM
Transformation
1 reply to this topic
#2
bob1
-
- Global Moderators
-
- 4,337 posts
Thelymitra pulchella
222
Excellent
Excellent
Posted 01 February 2013 - 02:55 PM
-20 will kill the bugs (freeze/thaw) unless you make a glycerol stock, in which case -80 is better. You can store them for a couple of days at 4 deg C, but the number of bacteria that survive drops off quickly. It is always much much much better to do the plating immediately after the recovery.
Also tagged with one or more of these keywords: Transformation
 |
Protocols and Techniques Forums →
Molecular Cloning →
Transformation colonies does not contain insertStarted by Guest_Sandy Fowler_* , 08 May 2013  transformation, Ligation, Cloning
|
|
|
|
Protocols and Techniques Forums →
Molecular Cloning →
Transformant RFP expressionStarted by Guest_tihong10_* , 25 Feb 2013 RFP, DH5a, Transformation
|
|
|
||
Protocols and Techniques Forums →
Molecular Biology →
Transformation problemStarted by Guest_Christi_* , 17 Feb 2013 transformation, dh5a, top10
|
|
|
||
Protocols and Techniques Forums →
General Lab Techniques →
Transformation or Ligation problemsStarted by Guest_JessiBelli89_* , 07 Feb 2013 Transformation, Ligation, TOP10 and 2 more...
|
|
|
||
Protocols and Techniques Forums →
Molecular Cloning →
Freeze transformation mixture?Started by Guest_tihong10_* , 28 Jan 2013 transformation, freezing
|
|
|
