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PCR


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#1 uaue

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Posted 15 January 2004 - 08:41 AM

im trying to amplify a 2kb and 548bp band from gDNA of mice but never got to see the big band. i dont understand what happened! ive tried different conditions even to the point of putting 2 minutes for the extension but nothing happened.can anyone explain to me what had just occured?

thanks.

#2 drcliquet

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Posted 16 January 2004 - 06:59 AM

For me PCR is always a question of "magic".
I've tryed to set up the right conditions for many different amplifications. When the PCR is "good", it works in many different conditions.
But when I get no result, in my experience, changing parameters is just a waste of time.
The best thing to do is design a new set of primers and start form zero!




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