Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Ligation protocol


  • Please log in to reply
1 reply to this topic

#1 drcliquet

drcliquet

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 14 January 2004 - 08:31 AM

Have you ever performed a ligation between two inserts and only a vector? I can first ligate one of the inserts and two days after ligate the second one but it would be useful to perform it in a single step.
Thank you!

#2 tfitzwater

tfitzwater

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 96 posts
3
Neutral

Posted 14 January 2004 - 03:55 PM

It is possible to perform a 3-way ligation, but the transformation efficiency for the correct product will be about 10-fold lower than a 2-way ligation. So you need to use competent cells that have relatively high transformation efficiency.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.