what is the formula to convert the RFU taken from a spectromax into ng/ul?
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#2
Curtis
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Metaller Scientist
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Posted 30 January 2013 - 06:57 PM
you would need standards.
#3
claritylight
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Posted 31 January 2013 - 04:43 AM
I have a standard curve. How do I determine the ng/ul from the RFU and standard curve?
#4
bob1
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Thelymitra pulchella
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Posted 31 January 2013 - 12:02 PM
You have the standards plotted on a graph - which should give you a line, find the equation of that line (be aware most of these sorts of things are not straight lines!)...
#5
claritylight
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Posted 01 February 2013 - 05:17 AM
I have the equation for the line. How do I now convert the RFU into the concentration now? What do I plug into the equation? Thanks.
Edited by claritylight, 01 February 2013 - 05:26 AM.
#6
bob1
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Thelymitra pulchella
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Posted 02 February 2013 - 12:14 AM
The equation should be something like x=ay+b where a and b are numbers, and y is the rfu, x will be the ng/ul amount. Solve for x (or find a graphing program that will do it for you).
