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formula to convert RFU to ng/ul

Relative Fluorescence

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#1 claritylight

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Posted 30 January 2013 - 03:50 PM

what is the formula to convert the RFU taken from a spectromax into ng/ul?

#2 Curtis

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Posted 30 January 2013 - 06:57 PM

you would need standards.

#3 claritylight

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Posted 31 January 2013 - 04:43 AM

I have a standard curve. How do I determine the ng/ul from the RFU and standard curve?

#4 bob1

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Posted 31 January 2013 - 12:02 PM

You have the standards plotted on a graph - which should give you a line, find the equation of that line (be aware most of these sorts of things are not straight lines!)...

#5 claritylight

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Posted 01 February 2013 - 05:17 AM

I have the equation for the line. How do I now convert the RFU into the concentration now? What do I plug into the equation? Thanks.

Edited by claritylight, 01 February 2013 - 05:26 AM.

#6 bob1

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Posted 02 February 2013 - 12:14 AM

The equation should be something like x=ay+b where a and b are numbers,  and y is the rfu, x will be the ng/ul amount.  Solve for x (or find a graphing program that will do it for you).