Hi! Would you please suggest some good 16S univerisal primers for qPCR? I have difficult finding one and need it very soon.
I have tried a 466bp one, and it maybe too big.
Thanks a lot!
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good 16S univ. primer for qPCR?
Started by joy123, Jan 29 2013 11:10 AM
3 replies to this topic
#2
Curtis
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Posted 29 January 2013 - 08:33 PM
check Qiagen website, you need shorter amplicons, below 150 bp is desirable.
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joy123
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Posted 30 January 2013 - 10:11 AM
Thanks! Do you mean search "16S" in the "QIAGEN Reference Database"? That comes only 3 articles that using their reagents. Do you mean Qiagen website have other better way to look for primers? I appreciate your help!
Curtis, on 29 January 2013 - 08:33 PM, said:
check Qiagen website, you need shorter amplicons, below 150 bp is desirable.
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Posted 30 January 2013 - 06:40 PM
yes, since you are doing qPCR you can use their Quantitect products. I used 2-3 years ago and they worked really well. Just search their website for 16s.
