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good 16S univ. primer for qPCR?


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#1 joy123

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Posted 29 January 2013 - 11:10 AM

Hi! Would you please suggest some good 16S univerisal primers for qPCR? I have difficult finding one and need it very soon.
I have tried a 466bp one, and it maybe too big.

Thanks a lot!

#2 Curtis

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Posted 29 January 2013 - 08:33 PM

check Qiagen website, you need shorter amplicons, below 150 bp is desirable.

#3 joy123

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Posted 30 January 2013 - 10:11 AM

Thanks! Do you mean search "16S" in the "QIAGEN Reference Database"? That comes only 3 articles that using their reagents. Do you mean Qiagen website have other better way to look for primers? I appreciate your help!

check Qiagen website, you need shorter amplicons, below 150 bp is desirable.



#4 Curtis

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Posted 30 January 2013 - 06:40 PM

yes, since you are doing qPCR you can use their Quantitect products. I used 2-3 years ago and they worked really well. Just search their website for 16s.




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