2 replies to this topic

[Vinnie]

Hopefully somebody could help me out...

I have labelled a membrane protein with a probe (atto655) and I want to know the protein concentration.

As the protein is a membrane protein I have detergent present (tritonx100) and so can't use Abs at 280 nm.

The dye absorbs between approx 550 and 700 so I can't use a bradford or BCA assay.

Is there any other assay I could use in this circumstance?

[neuron]

2D quant kit can be used, just check-

http://biology200.gs...nt protocol.pdf

[Vinnie]

neuron, on 27 January 2013 - 08:25 PM, said:

2D quant kit can be used, just check-

http://biology200.gs...nt protocol.pdf

Thank you!

I think this will work for me!

Edited by Vinnie, 28 January 2013 - 04:11 AM.