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Protein concentration of a labelled membrane protein - Protein and Proteomics - BioForum

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Protein concentration of a labelled membrane protein


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2 replies to this topic

#1 Vinnie

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Posted 27 January 2013 - 08:57 AM

Hopefully somebody could help me out...

I have labelled a membrane protein with a probe (atto655) and I want to know the protein concentration.

As the protein is a membrane protein I have detergent present (tritonx100) and so can't use Abs at 280 nm.

The dye absorbs between approx 550 and 700 so I can't use a bradford or BCA assay.

Is there any other assay I could use in this circumstance?

#2 neuron

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Posted 27 January 2013 - 08:25 PM

2D quant kit can be used, just check-

http://biology200.gs...nt protocol.pdf

#3 Vinnie

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Posted 28 January 2013 - 04:11 AM

2D quant kit can be used, just check-

http://biology200.gs...nt protocol.pdf


Thank you!

I think this will work for me!

Edited by Vinnie, 28 January 2013 - 04:11 AM.





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