Oil red O preparation
Posted 26 January 2013 - 04:52 AM
Very simple stuff but I am annoyed with it,
I am trying to stain lipid droplets in cells using Oil Red O.
I had a previous batch of the stock solution that was perfect, no aggregates, very clean, and nice staining but I have tried to prepare some new from powder (0,5g in 100mL isopropanol), heating overnight at 56C and filtering twice in whatman paper but even with this and after additional filtration, I get a lot of aggreagates on my cells when observing in microscope.
I prepare my working solution from stock (I tried 6:4 and 3:1 ORO/water ratio) used immediately with and without filtering before adding to the slides, I wash with water several times but it doesnt seem to help
The solution I make, 0,5g ORO in 100mL isopropanol, seems very dark compared to the previous one but I think this is the usual concentration though....
If anyone has a way of doing it that works, I'd be pleased to know about
Posted 28 January 2013 - 02:28 PM
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.