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How reliable is enterokinase digestion?

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#1 Suzanna



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Posted 25 January 2013 - 11:38 AM

Hi everyone

I have to purify a protein that has a great reputation for being protease sensitive. I have done an in silico analysis for enterokinase sensitivity of this protein but turns out it has no cleavage site. I could skip this step altogether if the expression vector did not add a 20 aa epitope after a His fusion tag and preceding the MCS. How specific is digestion with enterokinase ? please help



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