Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How reliable is enterokinase digestion?


  • Please log in to reply
No replies to this topic

#1 Suzanna

Suzanna

    member

  • Active Members
  • Pip
  • 10 posts
0
Neutral

Posted 25 January 2013 - 11:38 AM

Hi everyone

I have to purify a protein that has a great reputation for being protease sensitive. I have done an in silico analysis for enterokinase sensitivity of this protein but turns out it has no cleavage site. I could skip this step altogether if the expression vector did not add a 20 aa epitope after a His fusion tag and preceding the MCS. How specific is digestion with enterokinase ? please help

thanks,

Susie




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.