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Need help on STAT1-GFP nuclear translocation

STAT translocation IFN GFP

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15 replies to this topic

#16 bob1

bob1

    Thelymitra pulchella

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Excellent

Posted 04 February 2014 - 07:52 PM

I know this topic has gone cold but i was curious to find out whether Fresna-ni had managed to show translocation of Stat-1 by using the EGFP tagged plasmid from Addgene. I have tried the same approach IFN treating three different cell lines and i couldn't find any translocation taking place using a live cell confocal. I haven't sequenced the plasmid yet but I am curious to know if there is a problem with it..

You are relatively unlikely to get a response from Fresa-ni, as their last post was in this topic and they were last active on the 17th June last year.







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