Hello there. Im trying to culture the MRC-5 cell line. Im using DMEM + 10% FBS medium. After 5 days, the confluency only increased from 15% - 50% and there are still some cells floating. My questions are:
1. Why is this happening and how do I prevent it?
2. How long does it normaly takes for the number of MRC-5 cells to double?
3. What is the best protocol to detach the cells from the flask without damaging the cells?
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