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How To Culture MRC-5 cell line

MRC-5 growth time trypsinisation

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#1 youngpadawan

youngpadawan

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Posted 25 January 2013 - 02:18 AM

Hello there. Im trying to culture the MRC-5 cell line. Im using DMEM + 10% FBS medium. After 5 days, the confluency only increased from 15% - 50% and there are still some cells floating. My questions are:

1. Why is this happening and how do I prevent it?
2. How long does it normaly takes for the number of MRC-5 cells to double?
3. What is the best protocol to detach the cells from the flask without damaging the cells?




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