Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

iCycler Calibration Question

SYBR green iCycler

  • Please log in to reply
1 reply to this topic

#1 HOYAJM

HOYAJM

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 159 posts
12
Good

Posted 24 January 2013 - 07:18 AM

Hi, I need to calibrate an iCycler rt-pcr machine for SYBR green I use. The documentation is very vague for this and the iCycler calibration kit I have does not include a SYBR green standard. What do I use for the standard? is a standard (pure dye) even needed? The SYBR green that I have also has fluorescein in the mix.

If anyone uses a bio-rad iCycler or IQ5 with SYBR green and knows how to calibrate it, please let me know!

Thanks

#2 HOYAJM

HOYAJM

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 159 posts
12
Good

Posted 24 January 2013 - 08:14 AM

Bio-Rad did get back to me. To calibrate an iCycler for SYBR green, all you need to do is copy the .RME file for FAM and paste in the value for SYBR-490 in the .RME file in the program folder. You can do this since FAM and SYBR green use the same wavelengths.

It is not intuitive at all and they definitely don't mention this in the users manual.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.