Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Neomycin resistance sequence

Neomycin resistance

  • Please log in to reply
1 reply to this topic

#1 weswes



  • Members
  • Pip
  • 1 posts

Posted 24 January 2013 - 04:12 AM

Hi all,

I have a non-commerical plasmid which I am trying to figure out if it has a Neomycin(G-418) resistance cassette or not. I thought one strategy would be to align the nucleotide sequence of Neomycin with the plasmid's. Unless I am confused, I found more than one sequence for Neomycin resistance. Would anyone know which sequence exactly should I look for within my plasmid? or any other ideas?


#2 cellthetruth



  • Active Members
  • PipPipPipPipPip
  • 39 posts

Posted 24 January 2013 - 06:00 AM

Hi weswes,

Neomycin belongs to the group of aminoglycoside antibiotics, so does G-418 (also known as Geneticin). The "usual" Neo-resistance comes from an APH3' resistance cassette (phosphorylates the 3' OH in aminoglycosides). Pay attention that there are other resistance cassettes which confer resistance to several aminoglycosides such as APH2'', AAC2 etc. This means not all resistance cassettes confer resistance to the same aminoglycosides, e.g. Tobramycin has a 3' H and won't be phosphorylated by APH3' and remains active.
A good overview you can get in this Minireview: Aminoglycosides: Activity and Resistance. Mingeot-Leclercq et al., AAC 49(4) p. 727-737, April 1999

If you have the sequence of the Neomycin resistance cassette you should simply BLAST and you'll know immediatly which one is yours.


Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.