2 replies to this topic

[harry348]

what does 200 U 10 U/µl of not1 enzyme mean.
https://www.roche-ap...Id=3.6.1.1.68.1

does it mean every ul has 10U and if we want to use 1U we need to dilute it?

​Here im doing a double digestion with BamH1 and my band of interest  to be cut is 1800bp

Thanx in aadvance

[neuron]

Yes you are right, it means every ul contains 10 units of enzyme. For 1 U take 0.1ul.

[harry348]

thanx