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restriction enzyme


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#1 harry348

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Posted 24 January 2013 - 02:51 AM

what does 200 U 10 U/µl of not1 enzyme mean.
https://www.roche-ap...Id=3.6.1.1.68.1

does it mean every ul has 10U and if we want to use 1U we need to dilute it?

​Here im doing a double digestion with BamH1 and my band of interest to be cut is 1800bp

Thanx in aadvance

#2 neuron

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Posted 24 January 2013 - 03:13 AM

Yes you are right, it means every ul contains 10 units of enzyme. For 1 U take 0.1ul.

#3 harry348

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Posted 24 January 2013 - 06:02 AM

thanx




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