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[Tarheel Peach]

Hi,

I know this is a no brainer but I am struggling and the formula that I have been using I think is wrong.  I have used a hemacytometer and have calculated it this way before, but now I am using an automated cell counter and not sure if I am taking everything into consideration.  

I want to plate 1x10^5 cells into a T-25 flask with a final volume of 10 mls.  My cell sample is 20 ul into 20 ml of isoton (1/1000).  The cell counter accounts for this dilution.  I am trying to conduct proliferation experiments to determine doubling time.  Is there a specific formula to calculate this.

[bob1]

So the cell counter gives you a number of cells/ml?  If so you can use C1V1=C2V2 to do the calculation or c=n/v if you prefer