WB of cellular proteins in human serum
Posted 23 January 2013 - 11:11 AM
Any ideas of how to clean up these proteins?
Or is there an ELISA kit protocol for working with human serum?
Thanks in advance
Posted 23 January 2013 - 11:39 AM
IgG and other immunoglobulins can be removed by binding to protein A or G beads. Albumin I don't know, but you may be able to try something like a cibacron blue.
Posted 23 January 2013 - 11:53 AM
Trust me, I had enought trouble with this WB already, I have got results from a few blots and my PI wants me get this done, so I'm going to try the direct ELISA for this protein and check what I get.
I know the concentration of this protein compared to albumin or IgGs is ridiculous. But the cleaning spinning column didn't work at all.
I got a test kit to try this, I'll post here if I get any good results.