I have a 96 tandem array repeat clone but need half of them in new clone
Posted 22 January 2013 - 08:33 AM
Please help me in this
Posted 22 January 2013 - 11:31 AM
1) find a cut site in the array that will cut it in half more or less, blunt the cut site and do something similar for the other end of the plasmid.
2) PCR the array off the plasmid using primers with unique cutting site incorporated.
Posted 22 January 2013 - 11:51 AM
Posted 04 February 2013 - 05:24 AM
Thanks for the reply...The 96binding site tandem is developed by biobrick cloning and it took long time for us..Thanks however
Thelymitra...can you please elaborate it to more extent
My Plasmid sequence is RFP1-96 BS(ACATGAGGATCACCCATGTGT)96 times....can you please explain furhter so that i could design primers...Thanks
Posted 04 February 2013 - 06:23 AM