2 replies to this topic

[sabi67]

Hi evryone,

I'm doing a 5ml liquid culture of DH5 E.coli transformed with an ampicillin resistance plasmid. I'd like to have a culture that  grew 12 to 16h in LB before doing the miniprep but that is really not fitting well my planning. I was wondering if it's OK to pause the culture by placing the tube at 4°C and then replace them at 37°C ?
WIll this step have any consequence on the bacterial growth or the plasmid expression?

Thanks in advance for your answers!

Sabrina

[HOYAJM]

Sure, you can do that. It seems that you are worried about it growing too long. You can do minipreps from 5mL cultures that grew for 22h without a problem. Either way, you shouldnt have any issues

[sabi67]

Thanks a lot!