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Can I pause a bacterial culture at 4C


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#1 sabi67

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Posted 22 January 2013 - 06:39 AM

Hi evryone,

I'm doing a 5ml liquid culture of DH5 E.coli transformed with an ampicillin resistance plasmid. I'd like to have a culture that grew 12 to 16h in LB before doing the miniprep but that is really not fitting well my planning. I was wondering if it's OK to pause the culture by placing the tube at 4°C and then replace them at 37°C ?
WIll this step have any consequence on the bacterial growth or the plasmid expression?

Thanks in advance for your answers!

Sabrina

#2 HOYAJM

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Posted 22 January 2013 - 08:38 AM

Sure, you can do that. It seems that you are worried about it growing too long. You can do minipreps from 5mL cultures that grew for 22h without a problem. Either way, you shouldnt have any issues

#3 sabi67

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Posted 23 January 2013 - 08:04 AM

Thanks a lot!




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