Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Lipid extraction from parrafin embedded tissue ?

  • Please log in to reply
1 reply to this topic

#1 julnobody



  • Active Members
  • Pip
  • 18 posts

Posted 21 January 2013 - 10:11 AM


I am planning on doing lipid analysis on cells, tissue (fresh frozen) and parrafin embedded.

Among other things I want to perform a cholesterol level measurement, is it possible to do so by lipid extraction from formalin-fixed parrafin-embedded tissue ? (Fresh frozen is probably the best but I have more material parrafin embedded)

Thank you;

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,512 posts

Posted 21 January 2013 - 11:34 AM

Unfortunately for you, the standard process for paraffin embedding is to dehydrate the tissue first (paraffin is immiscible with water). This is most commonly done in a series of graded alcohols followed by xylene, which will dissolve the fats.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.