1 reply to this topic

[julnobody]

Hi,

I am planning on doing lipid analysis on cells, tissue (fresh frozen) and parrafin embedded.

Among other things I want to perform a cholesterol level measurement, is it possible to do so by lipid extraction from formalin-fixed parrafin-embedded tissue ? (Fresh frozen is probably the best but I have more material parrafin embedded)

Thank you;

[bob1]

Unfortunately for you, the standard process for paraffin embedding is to dehydrate the tissue first (paraffin is immiscible with water).  This is most commonly done in a series of graded alcohols followed by xylene, which will dissolve the fats.