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Lipid extraction from parrafin embedded tissue ?


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#1 julnobody

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Posted 21 January 2013 - 10:11 AM

Hi,

I am planning on doing lipid analysis on cells, tissue (fresh frozen) and parrafin embedded.

Among other things I want to perform a cholesterol level measurement, is it possible to do so by lipid extraction from formalin-fixed parrafin-embedded tissue ? (Fresh frozen is probably the best but I have more material parrafin embedded)

Thank you;

#2 bob1

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Posted 21 January 2013 - 11:34 AM

Unfortunately for you, the standard process for paraffin embedding is to dehydrate the tissue first (paraffin is immiscible with water). This is most commonly done in a series of graded alcohols followed by xylene, which will dissolve the fats.




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