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How long can I store cell lysis buffer at 4C for?

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#1 science noob

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Posted 20 January 2013 - 10:41 PM

Is it possible to keep the lysis buffer (containing protease + phosphatase inhibitors) for more than a week?
How long (if any) does it take for these inhibitors to degrade?

#2 bob1


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Posted 20 January 2013 - 11:55 PM

Some of these inhibitors will degrade very rapidly (can't recall which ones specifically atm), so storage in the liquid form for more than a week is not a good idea. You can store frozen inhibitors for up to about 6 months and lysis buffer without inhibitors for a similar period at 4 deg C.

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