Is it possible to keep the lysis buffer (containing protease + phosphatase inhibitors) for more than a week?
How long (if any) does it take for these inhibitors to degrade?
Submit your paper to J Biol Methods today!
1 reply to this topic
#2
bob1
-
- Global Moderators
-
- 6,387 posts
Thelymitra pulchella
512
Excellent
Excellent
Posted 20 January 2013 - 11:55 PM
Some of these inhibitors will degrade very rapidly (can't recall which ones specifically atm), so storage in the liquid form for more than a week is not a good idea. You can store frozen inhibitors for up to about 6 months and lysis buffer without inhibitors for a similar period at 4 deg C.
