3 replies to this topic

[lyok]

Hallo all,
I recentely received some plasmids. Now I am going to sequence them because the sequence is not know. What should I do after I received the sequences? Can I post/upload them somewhere?
I know I can do this, but the thing is: the plasmids are not really mine... So would this still be ok?
The person that gave them to me is also not really the owner, he also got them from someone else...

[phage434]

You can upload them to Genbank, but they will refuse to add any references to publications on the plasmid -- only the original authors can deposit sequence with reference. I still think this is very worthwhile. Others will thank you.

[lyok]

phage434, on 19 January 2013 - 01:30 PM, said:

You can upload them to Genbank, but they will refuse to add any references to publications on the plasmid -- only the original authors can deposit sequence with reference. I still think this is very worthwhile. Others will thank you.

Well, since I dont even know the original owners of the plasmid, I wouldnt even send them a name/paper. You couldnt even find most of the plasmids if you did a websearch anyway...          

But I'll still be able to just send them the plasmid sequences then? ANd would it have my name then as the one who uploaded it? or?
I have never done this before and dont really know anything about it.
The only reason I was thinking about doing this is because I was frustrated myself that the sequences were not known at the first place (I find this a bit strange to be honest, that the plasmids are being used and not sequenced) so that in the future others might be helped.

[phage434]

Yes, your name would be in the sequence record.  See here for insructions:
http://www.ncbi.nlm....genbank/submit/