Dear All,
After I got my samples ready (quite large samples) for cell cycle analysis I found the FACS is not working I got very upset because I spent long time preparing these samples. Actually I didnt know what is the proper thing I should do with the samples especially because I was working in weekend so I decided to put the samples in -80 (samples labeled with PI) ....
Do you think it is possible to store labeled samples??
Please please offer me all the help you can...
Many thanks in advance
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Heeeeeelp..cell cycle sample..is it possible to store it?
Started by shs, Jan 19 2013 09:45 AM
FACS cell cycle
2 replies to this topic
#1
shs
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Posted 19 January 2013 - 09:45 AM
Science is What We Need To Understan The Life
#2
bob1
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Thelymitra pulchella
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Excellent
Excellent
Posted 19 January 2013 - 03:37 PM
It should be fine to store at 4 deg C if they are fixed, you may need to re-label the samples with PI though. -80 will probably cause the cells to be too damaged by ice crystal formation during the freeze/thaw process, to be used.
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Posted 20 January 2013 - 01:26 PM
Thank you so much for ur replay. So I need to prepare the samples again 
Science is What We Need To Understan The Life
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