I isolated RNA from human tissue samples using RNeasy.
I electrophoresed the RNA obtained and this is what I got:
P.S. 100bp DNA ladder was used instead of RNA ladder because we don't have RNA ladder in our lab.
From the gel image, two clear bands can be seen (supposed to be 18S and 28S rRNA).
But there are also some smaller bands down there.
Does this indicate RNA degradation or something else?
P.S. The tissue samples were preserved in RNAlater for 1 month before RNA isolation.
P.S. We don't have Bioanalyzer in the lab, so I cannot analyse it in the instrument.
P.S. I did not check the purity and concentration of the RNA because the only spectrophotometer in our lab has been send for repair.
Edited by jamestoon1, 19 January 2013 - 09:46 AM.