I need help for designing a pair of primers for this sequence.Primer designing
Posted 18 January 2013 - 07:00 PM
My Protein of interest binds to /cagagtggcctcaaactc/ and I want a pair of primers for Chromatin immunoprecipitation-qPCR for this sequence below.
I have used Softwares (Oligo, DNAstar) and online sites like NCBI and Primer3 (Plus) to automaticly find primers for me, but whenever I check them in https://www.ncbi.nlm...s/primer-blast/
They bind to more than one places. I do not have too knowledge to choose primers manually.
Could you help me?
Posted 21 January 2013 - 11:27 AM
Filter out all those and select the best primer pair from those you designed.
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