Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

General chat about tween-20 as a WB block and wash

western blot tween 20 wash block antibody

  • Please log in to reply
1 reply to this topic

#1 Joe101

Joe101

    member

  • Active Members
  • Pip
  • 7 posts
0
Neutral

Posted 18 January 2013 - 04:01 AM

Hi,

I was interested about the specific mechanism by which tween-20 works as a block and wash of western blot membranes, but unfortunately I couldn't find much on it except for one bulletin. This suggested the large size of tween-20 would make it useful for a block but also that it's size is due to it's large hydrophillic head so that doesn't quite make sense. As for it's ability to wash the membrane of antibodies, tween-20 has a low CMC and so may incorporate loosely bound antibodies into mixed micelles, while leaving bigger more strongly bound proteins on the membrane.

I dont know though... any ideas?

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,664 posts
394
Excellent

Posted 18 January 2013 - 02:29 PM

It doesn't function as a block at all - this is entirely down to the protein (e.g. milk powder) that you put in the block. The Tween acts largely as a surfactant to ensure that the whole surface of the membrane is coated and stays coated. It also acts as a stringency agent, stopping (mainly) non-specific binding by causing the antibodies to be in a partially denatured state.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.