Treatment 1 (Sample 1) 0.390 , 0.374 (Sample 2)0.439, 0.458 (Sample 3)0.441, 0.444
Treatment 2 (Sample 1) 0.418 , 0.424 (Sample 2)0.446, 0.471 (Sample 3)0.406, 0.436
+ Control (Sample 1) 0.428 , 0.454 (Sample 2)0.429, 0.432 (Sample 3)0.441, 0.427
My cells are adherent cancer cells, seeded in 24-wells at 2500 cells/well and harvested on the 5th day of seeding.
Honesyly, I don't see much difference in cell density when view under microscope across all the wells.
Those data were obtained after incubation with MTT for about 2 hours, and solublised with DMSO for about 10 minutes with swirling.
The samples were then transfered to 96-wells plate for reading ( I pipet up and down)
But irrespective of that, I am expecting to get some of the treatment and + control to have about 1.5 fold increases over control... but the well-to-well variations of the same treatment set are quite big..I tried to avoid pipeting errors already..
Is there really serious problem with my pipeting? or could it be due to other factors? I have repeated the experiments several times and still don't get it work out.. What can I try to do next?
Please help... it's for my final year project which has very very tight schedule from now onwards... thx thx thx
Edited by hanazawayien, 18 January 2013 - 01:58 AM.