Change in Superscript lot influences transcript level of one gene but not others
Posted 18 January 2013 - 12:14 AM
Posted 21 January 2013 - 11:40 AM
If not, there is possibility the transcript selectively degraded (that can happen) before the switch.
You can always try a third RT system (side by side) to check if there is variability among different RTs.
I never trust anything that can't be doubted.
Posted 21 January 2013 - 12:04 PM
I think the only option will be to order a new lot of this kit and hope this is working more similar to the old lot than the one we have now. I'm working in a diagnostics lab and it's not so easy to change protocols, it requires extensive validation. One normally hopes/sees that different lot numbers behave similarly, but apparently that's not always the case.