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blood contamination in tissue

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#1 Xin



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Posted 17 January 2013 - 10:59 AM

Hi, Friends

I have been trying to isolate proteins and RNAs from mice hearts and other tissues but there seems to be a fairly amount of contamination with blood. I am not sure whether this will affect my results or not. Also, is there a way to reduce this contamination?

Thanks a lot.

#2 bob1


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Posted 17 January 2013 - 11:36 AM

It may affect your results depending on what you are looking for. Before/while you euthanise the mouse you can perfuse with PBS or something similar to wash out the blood.

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