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Incomplete digestion of plasmid with single enzyme

how many bands?

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#1 neuron

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Posted 17 January 2013 - 03:41 AM

If we load uncut plasmid on agarose gel, we see 3-5 bands. But if the plasmid is digested with one enzyme and apart from the expected band you see very faint other bands also . Does that mean that the plasmid is still uncut and it shows the other forms on gel?

#2 bob1

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Excellent

Posted 17 January 2013 - 11:56 AM

probably




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