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PCR problem


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#1 xunfen

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Posted 08 January 2004 - 12:04 PM

I tried different concentration of DNA template, different Taq, most of time i only get huge band larger than 2000bp, soetime got 500 bp band, while i am looking forward the 200bp band.
what happened?

#2 pcrman

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Posted 08 January 2004 - 12:17 PM

could you provide more info about your pcr? What is your template? genomic PCR or RT-PCR?




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