Has anyone tried to crosslink 3-4 proteins and inject in mice or rabbit?
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#2
Curtis
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Posted 19 January 2013 - 09:33 AM
what is the use of the generated antibody? I'm curious. Does it bind to all the proteins?
#3
neuron
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Posted 19 January 2013 - 10:40 PM
I also don't know. It should not bind
. I was wondering if somebody has tried generating antibody against a complex. I asked this question in another thread also-
http://www.protocol-...rotein-complex/
As chelo mentioned that it has been done, I dint find any publication on that. And as crosslinkers are available to capture the protein interactions. I was thinking is it possible to generate antibodies for two proteins by crosslinking them?
. I was wondering if somebody has tried generating antibody against a complex. I asked this question in another thread also-http://www.protocol-...rotein-complex/
As chelo mentioned that it has been done, I dint find any publication on that. And as crosslinkers are available to capture the protein interactions. I was thinking is it possible to generate antibodies for two proteins by crosslinking them?
