Has anyone tried to crosslink 3-4 proteins and inject in mice or rabbit?
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crosslinking of protein and generate antibody
Started by neuron, Jan 16 2013 11:36 PM
2 replies to this topic
#1
Posted 16 January 2013 - 11:36 PM
#2
Posted 19 January 2013 - 09:33 AM
what is the use of the generated antibody? I'm curious. Does it bind to all the proteins?
#3
Posted 19 January 2013 - 10:40 PM
I also don't know. It should not bind
. I was wondering if somebody has tried generating antibody against a complex. I asked this question in another thread also-
http://www.protocol-...rotein-complex/
As chelo mentioned that it has been done, I dint find any publication on that. And as crosslinkers are available to capture the protein interactions. I was thinking is it possible to generate antibodies for two proteins by crosslinking them?

http://www.protocol-...rotein-complex/
As chelo mentioned that it has been done, I dint find any publication on that. And as crosslinkers are available to capture the protein interactions. I was thinking is it possible to generate antibodies for two proteins by crosslinking them?
