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Stable cell lines using Flp-In


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#1 zingmatter

zingmatter

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Posted 08 January 2004 - 06:28 AM

Hi

I want to create a cDNA library that is stably transfected into HEK 293 cells using Invitrogen's Flp-In kit.

My problem is that I need to know what percentage of integration I can see (using the pre-made FRT carrying cells). If the percenatage of cells that integrate DNA is not good then I'd be struggling to get a full representation of the cDNA library.

My alternative is to use a retroviral method but I don't want to go down that route if I can avoid it.

If anyone has any experience with Flp technology I appreciate some feed back.

Thanks

zing




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