Blocking solution mistake, please help!
Posted 15 January 2013 - 05:56 PM
This is a quick question, for my blocking step in western blotting I used 5% skim milk. Instead of dissolving the skim milk in 1X TBST, I made a mistake and dissolved it in 1X TBST containing 5% BSA (which I normally use to dilute my antibodies). I went on and incubated the membrane overnight with primary antibody. I can't stop thinking about, I am worried, will my mistake affect my result? Please help!
Posted 16 January 2013 - 12:09 AM
Posted 16 January 2013 - 08:08 AM