Posted 15 January 2013 - 06:41 AM
Thank you in advance,
Posted 15 January 2013 - 08:08 AM
does your loading buffer contain glycerol or sucrose?
are you sure you diluted the electrode buffer to 1x?
my guess is that glycerol from the running gel is leaching into the wells so a flush prior to loading should clear the problem.
Edited by mdfenko, 15 January 2013 - 08:21 AM.
talent does what it can
genius does what it must
i used to do what i got paid to do
Posted 18 January 2013 - 05:02 PM