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Sanger Sequencing RNAse Interference?

RNAse sanger sequencing

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#1 Dino

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Posted 14 January 2013 - 05:49 PM

Hi everyone,

Just wondering for those who have experience with sequencing. Does having RNAseI in your plasmid preps affect sequencing?

I have a bit of contam in my samples and a little paranoid it will not give me clean sequencing.

Thanks!
D

#2 phage434

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Posted 14 January 2013 - 06:22 PM

RNAse has no effect on sequencing (other than the normal issues of having too much protein in a sequencing reaction). The RNAse should be largely removed by later stages of a miniprep procedure.





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