Hi everyone,
Just wondering for those who have experience with sequencing. Does having RNAseI in your plasmid preps affect sequencing?
I have a bit of contam in my samples and a little paranoid it will not give me clean sequencing.
Thanks!
D
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1 reply to this topic
#2
phage434
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Posted 14 January 2013 - 06:22 PM
RNAse has no effect on sequencing (other than the normal issues of having too much protein in a sequencing reaction). The RNAse should be largely removed by later stages of a miniprep procedure.
Also tagged with one or more of these keywords: RNAse, sanger, sequencing
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