Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Protein Expression vector with small tag


  • Please log in to reply
1 reply to this topic

#1 kirstykay

kirstykay

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 14 January 2013 - 08:38 AM

I'm looking for a bacterial vector to over express my protein. Ideally with a smaller tag than His of Gst (at the N-terminal). Does anyone know of any please. I want to use my protein in enzyme activity assays once made.

Many thanks
Kirsty

#2 HOYAJM

HOYAJM

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 159 posts
12
Good

Posted 14 January 2013 - 09:00 AM

Why don't you use a cleavable His tag? That way you have no tag at all after purification/cleavage. look into the pET vectors if you are interested in that.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.