I'm going to start to work in chondrogenic differentiation of a mouse bone marrow MSCs immortalized cell line. We still have a lot of human TFG-beta1 from other works. My doubt is about using this human factor in mouse cells. I have seen in blastp that the translated sequence for the mouse mRNA have a big match with the human one, but it also has with human TGF-beta3. Any advice, please? If i could use the growth factor it woul be great, because it's an expensive material.
Edited by Lord Apoptosis, 14 January 2013 - 08:46 AM.