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Tissue Homogenate


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#1 Shannah

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Posted 14 January 2013 - 12:49 AM

I'll have to make a homogenate of tissue (aorta) and now I face the problem what the best method to store them.
I cannot not use them immediatly so I have to collect them. Is it better to store the aorta as whole or as homogenates? I'm a little bit afraid of protein degredation because I have to measure different protein levels of the homogenates ;)

Thanks for your help.

#2 bob1

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Posted 14 January 2013 - 12:00 PM

Either should be fine at -80, but for best results I would homogenize in a buffer with protease inhibitors and then freeze.




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