I've recently started my PhD in a lab where there is a massive problem with mycoplasma. The majority of cell lines in the lab that have been tested have been shown to be positive, including some of mine that I imported from overseas (although I think that they may have been contaminated before I even got them ).
The head of my lab just doesn't want to hear about it- it has been brought up several times with him and he dismisses it (he is a bit of a tyrant). He does not want us to throw out contaminated cells, and says that even testing for mycoplasma is a waste of time. I know this is wrong, and my results have been affected by the mycoplama infection in the lines that I'm using.
I guess what I'm asking is this: if I bring up new, uncontaminated stocks of cells, what are the chances that they will catch mycoplama from being in an incubator/hood with other infected cell lines?
And furthermore, is there any way, or has anyone had any success, in curing cells of a mycoplama infection? I have read some reports of it being possible, and have attached a protocol here that I was considering trying as throwing things out is apparently not an option in this lab.
Very worried PhD candidate