Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How to raise polyclonal antibodies against whole bacteria live &/ or inactiv


  • Please log in to reply
4 replies to this topic

#1 Inbox

Inbox

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 328 posts
21
Excellent

Posted 12 January 2013 - 10:21 AM

can you please suggest protocol for raising polyclonal antibodies against whole bacteria live &/ or inactivated.

Thanks

#2 Pangea

Pangea

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 97 posts
7
Neutral

Posted 12 January 2013 - 11:50 AM

In which Organism will you perform your experiment? Hamster, Mouse, Rabbit etc.? Can be tricky with polyconal, am I wrong?

#3 Inbox

Inbox

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 328 posts
21
Excellent

Posted 12 January 2013 - 01:53 PM

Rabbit. I'm not familiar with protocol in hamster, mouse, but I guess more or less same principle- dose then booster at intervals.

Edited by prabhubct, 12 January 2013 - 02:00 PM.


#4 Pangea

Pangea

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 97 posts
7
Neutral

Posted 13 January 2013 - 06:41 AM

Thats just general information, but which bacteria you want to produce rabbit Ab. It will be take 3 month i guess in rabbit. Whole bacteria means just its capsule, isnt? You might look up the consistence of the capsule. It dosent matter if it Gram-negative or -positive, isnt? But i guess it will be unspecific Antibody. Do your Bacteria has specific marker molecules on his surface?

http://www.uniprot.org/keywords/875

http://ecvam.jrc.ec....hopReport35.pdf

#5 Inbox

Inbox

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 328 posts
21
Excellent

Posted 24 January 2013 - 05:52 AM

Thanks & sorry for late reply, little busy couldn't visit forum for some days. Its Salmonella typhimurium.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.