Hi I was wondering what voltage/time others have used with PVDF membranes to transfer proteins of this size efficiently? Thanks.
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Wet transfer of proteins between 100-200kDa
Started by
LacquerHead
, Jan 11 2013 10:19 AM
2 replies to this topic
#2
Pangea
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Posted 12 January 2013 - 12:01 PM
I think 120V for 1.30 - 2.00 h. Maybe should put PVDF in methanol first before transferring. Always check your protein ladder/marker.
#3
bob1
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Posted 14 January 2013 - 12:05 PM
Low and slow is always best for a wet transfer. I use 15-30 V overnight. Higher voltages cause problems with heating. Minimize the amount of methanol you use and you can add some SDS to increase mobility.
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