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Wet transfer of proteins between 100-200kDa
2 replies to this topic
Posted 11 January 2013 - 10:19 AM
Hi I was wondering what voltage/time others have used with PVDF membranes to transfer proteins of this size efficiently? Thanks.
Posted 12 January 2013 - 12:01 PM
I think 120V for 1.30 - 2.00 h. Maybe should put PVDF in methanol first before transferring. Always check your protein ladder/marker.