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Wet transfer of proteins between 100-200kDa


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#1 LacquerHead

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Posted 11 January 2013 - 10:19 AM

Hi I was wondering what voltage/time others have used with PVDF membranes to transfer proteins of this size efficiently? Thanks.

#2 Pangea

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Posted 12 January 2013 - 12:01 PM

I think 120V for 1.30 - 2.00 h. Maybe should put PVDF in methanol first before transferring. Always check your protein ladder/marker.

#3 bob1

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Posted 14 January 2013 - 12:05 PM

Low and slow is always best for a wet transfer. I use 15-30 V overnight. Higher voltages cause problems with heating. Minimize the amount of methanol you use and you can add some SDS to increase mobility.




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