We have a pcr/gel-purification column kit that I could try to use to purify the MCS piece from the vector but I don't know how effective that is. It claims it removes DNA fragments less than 100bp, but how efficient is it? Am I better off gel purifying it, or will that just potentially risk degrading it if I have to use UV light? Of course, if the restriction digest doesn't look complete, I should gel purify it anyway to get rid of uncut vector, but I'm optimizing the digestion reaction now. Are there any papers out there showing the percent transformants of MCS versus insert after a double digest?
Edited by assembler01, 11 January 2013 - 09:11 AM.