1 reply to this topic

[Hemavet]

Hi,

I have 250 Bovine serum samples. I would like to remove Albumin from these samples to have the chance to visualize the low abundant Immunoglobulin (IgA and IgM) by FT-IR.
If anyone know protocol to precipitate or remove albumin from these sample just write it to me.

Thanks,
Hema

[Pangea]

You can use Affinity Chromatography or Ion Exchange Chromatography (Isoelectric Point). If you have less amount maybe with sepharose beads.