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Albumin Removal From BSA


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#1 Hemavet

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Posted 10 January 2013 - 01:40 PM

Hi,

I have 250 Bovine serum samples. I would like to remove Albumin from these samples to have the chance to visualize the low abundant Immunoglobulin (IgA and IgM) by FT-IR.
If anyone know protocol to precipitate or remove albumin from these sample just write it to me.

Thanks,
Hema

#2 Pangea

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Posted 12 January 2013 - 12:22 PM

You can use Affinity Chromatography or Ion Exchange Chromatography (Isoelectric Point). If you have less amount maybe with sepharose beads.




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