Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

can restriction enzymes be stored at -70C?

restriction enzyme

  • Please log in to reply
3 replies to this topic

#1 sorcener

sorcener

    member

  • Active Members
  • Pip
  • 13 posts
0
Neutral

Posted 09 January 2013 - 03:33 PM

Hi everyone,

I'm having troubles with my -20° C freezer, it works fine for a time but sudenly heats! I have restriction enzymes enzymes, PCR stuff, some antibodies and DNA samples. It have been "fixed" several tinmes but still the same =(, and boss says there's no money so we always are running to store things in other labs which is really impractical for work.

we hace a -70°C Revco and i now i can store perfectly fine my DNA samples there, but i don't know in the case of enzymes. My logic says it would be fine but want to be sure.

Does enyone knows or have done ir before?

Manu thanks in advance

#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,484 posts
251
Excellent

Posted 09 January 2013 - 07:06 PM

A few freeze/thaw cycles would be fine, but the glycerol in the storage buffer is intended to keep the enzyme liquid at -20, to eliminate the need for freeze/thaw cycles in everyday use. Our REs are shipped on dry ice, which is at -80.

#3 sorcener

sorcener

    member

  • Active Members
  • Pip
  • 13 posts
0
Neutral

Posted 10 January 2013 - 07:25 AM

so i could make alicuots and store them at -70°C so when i took one out to use i would not freeze it again and not spoil it?

#4 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,484 posts
251
Excellent

Posted 10 January 2013 - 08:02 AM

Yes, that would be fine.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.